Journal: Frontiers in Neuroscience

Article Title: Long-term aerobic exercise improves learning memory capacity and effects on oxidative stress levels and Keap1/Nrf2/GPX4 pathway in the hippocampus of APP/PS1 mice

doi: 10.3389/fnins.2024.1505650

Figure Lengend Snippet: Aerobic exercise regulates Keap1/Nrf2/GPX4 pathway of the hippocampus in APP/AS1 mice. (A) Representative western blots of Keap1, Nrf2, HO-1, and GPX4 expression. (B–E) Quantification data of Keap1, Nrf2, HO-1, and GPX4 protein expression. n = 4, ∗ p < 0.05, compared with the control group; # p < 0.05, compared with the model group; & p < 0.05, compared with the S-te group. C, control group; M, model group; S-te, short-term exercise group; L-te, long-term exercise group.

Article Snippet: The protein sample was then electrophoresed in 12% SDS-polyacrylamide gels, transferred onto the PVDF membrane, and blocked using 5% skimmed milk for 1 h. The membrane was incubated with diluted (1:1000) primary antibodies against Keap-1 antibody (ab178846; Abcam), Nrf2 antibody 28,205-1-AP; Proteintech, HO-1 antibody (13129-1-AP; Proteintech, Shanghai, China), or GPX4 antibody (14104-1-AP; Proteintech, Shanghai, China) at 4°C overnight.

Techniques: Western Blot, Expressing, Control

Journal: Annals of Clinical and Translational Neurology

Article Title: A novel de novo GABRA2 gene missense variant causing developmental epileptic encephalopathy in a Chinese patient

doi: 10.1002/acn3.52262

Figure Lengend Snippet: Analysis of GABA A R pentameric structure. (A) A pentameric model featuring 2 α2 subunits (GABRA2), 2 β3 subunits (GABRB3), and 1 γ2 subunit (GABRG2). (B) The structure is rotated 90 degrees for a top‐down view. In the wild‐type GABRA2, Ala308 forms hydrogen bonds with Pro209 of GABRB3 and with another GABRA2 protein at position 238 of GABRG2. (C) In the mutated GABRA2 (p.Ala308Val), the longer valine side chain disrupts these hydrogen bonds, potentially altering structural stability.

Article Snippet: The primary antibodies utilized included: DYKDDDDK Tag (9A3) Mouse mAb (Cat#8146, Cell Signaling Technology, MA, USA, diluted 1:200); GABRB3 Antibody (Cat#PB0627, Boster Biological Technology, Wuhan, China, diluted 1:1000); GABRG2 Antibody (Cat#A02361‐1, Boster Biological Technology, Wuhan, China, diluted 1:1000); and β‐Actin (8H10D10) Mouse mAb (Cat#3700, Cell Signaling Technology, MA, USA, diluted 1:1000).

Techniques:

Journal: Annals of Clinical and Translational Neurology

Article Title: A novel de novo GABRA2 gene missense variant causing developmental epileptic encephalopathy in a Chinese patient

doi: 10.1002/acn3.52262

Figure Lengend Snippet: In vitro functional assay. (A) Western blotting analysis indicated that the GABRA2‐MUT group exhibited protein expression, but it was significantly lower than the GABRA2‐WT group, with a reduction of approximately 70.62% (*** P < 0.001). (B) CO‐IP results showed that wild‐type GABRA2 interacted with GABRB3/GABRG2, while the mutant may disrupt this interaction. The right panel presents normalized CO‐IP analysis, *** P < 0.001.

Article Snippet: The primary antibodies utilized included: DYKDDDDK Tag (9A3) Mouse mAb (Cat#8146, Cell Signaling Technology, MA, USA, diluted 1:200); GABRB3 Antibody (Cat#PB0627, Boster Biological Technology, Wuhan, China, diluted 1:1000); GABRG2 Antibody (Cat#A02361‐1, Boster Biological Technology, Wuhan, China, diluted 1:1000); and β‐Actin (8H10D10) Mouse mAb (Cat#3700, Cell Signaling Technology, MA, USA, diluted 1:1000).

Techniques: In Vitro, Functional Assay, Western Blot, Expressing, Co-Immunoprecipitation Assay, Mutagenesis